No, all your good work hasn't been undone. But, yes, consuming that much gluten in one meal (or even over a period of days) is going to make you feel like sh*t.
When it comes to wheat, barley, rye... strive for 100% because there is still going to be trace gluten on everything -- especially if you eat out. Those proteins are sticky suckers, and they can cause autoimmune responses well after ingesting them.
I am a big fan of cheating, but I won't cheat with wheat. It makes me feel lousy.