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    jqbancroft's Avatar
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    Article Abstract: Is this saying what I think it's saying?

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    My aunt, who used to be a nursing professor, asked for some research behind the whole grains as anti-nutrients thing. I was doing some research with my University's library, and I came accross this abstract. It's a little too "sciency" for me to fully grasp, but I think their conclusion is that the lectins in wheat interfere with our gastrointestinal functioning. But I'm not sure. Haha. I'll paste it below if you don't mind telling me which side they are on:

    "Wheat germ agglutinin (WGA) is a plant protein that binds specifically to sugars expressed, among many others, by human gastrointestinal epithelial and immune cells. WGA is a toxic compound and an anti-nutritional factor, but recent works have shown that it may have potential as an anti-tumor drug and as a carrier for oral drugs. To quantitate the toxicity threshold for WGA on normal epithelial cells we previously investigated the effects of the lectin on differentiated Caco2 cells, and showed that in the micromolar range of concentrations WGA could alter the integrity of the epithelium layer and increase its permeability to both mannitol and dextran. WGA was shown to be uptaken by Caco2 cells and only [approximately equal to]0.1% molecules were observed to cross the epithelium layer by transcytosis. Here we show that at nanomolar concentrations WGA is unexpectedly bioactive on immune cells. The supernatants of WGA-stimulated peripheral blood mononuclear cells (PBMC) can alter the integrity of the epithelium layer when administered to the basolateral side of differentiated Caco2 cells and the effects can be partially inhibited by monoclonal antibodies against IL1, IL6 and IL8. At nanomolar concentrations WGA stimulates the synthesis of pro-inflammatory cytokines and thus the biological activity of WGA should be reconsidered by taking into account the effects of WGA on the immune system at the gastrointestinal interface. These results shed new light onto the molecular mechanisms underlying the onset of gastrointestinal disorders observed in vivo upon dietary intake of wheat-based foods."

    Effects of wheat germ agglutinin on human gastrointestinal epithelium: Insights from an experimental model of immune/epithelial cell interaction. Toxicology and Applied Pharmacology. June 1, 2009 v237 i2 p146(8). Authors: Chiara Dalla Pellegrina (a), Omar Perbellini (b), Maria Teresa Scupoli (b)(c), Carlo Tomelleri (b), Chiara Zanetti (a), Gianni Zoccatelli (a), Marina Fusi (a), Angelo Peruffo (a), Corrado Rizzi (a), Roberto Chignola (a)

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    Katt's Avatar
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    Quote Originally Posted by jqbancroft View Post
    My aunt, who used to be a nursing professor, asked for some research behind the whole grains as anti-nutrients thing. I was doing some research with my University's library, and I came accross this abstract. It's a little too "sciency" for me to fully grasp, but I think their conclusion is that the lectins in wheat interfere with our gastrointestinal functioning. But I'm not sure. Haha. I'll paste it below if you don't mind telling me which side they are on:

    "Wheat germ agglutinin (WGA) is a plant protein that binds specifically to sugars expressed, among many others, by human gastrointestinal epithelial and immune cells. WGA is a toxic compound and an anti-nutritional factor, but recent works have shown that it may have potential as an anti-tumor drug and as a carrier for oral drugs. To quantitate the toxicity threshold for WGA on normal epithelial cells we previously investigated the effects of the lectin on differentiated Caco2 cells, and showed that in the micromolar range of concentrations WGA could alter the integrity of the epithelium layer and increase its permeability to both mannitol and dextran. WGA was shown to be uptaken by Caco2 cells and only [approximately equal to]0.1% molecules were observed to cross the epithelium layer by transcytosis. Here we show that at nanomolar concentrations WGA is unexpectedly bioactive on immune cells. The supernatants of WGA-stimulated peripheral blood mononuclear cells (PBMC) can alter the integrity of the epithelium layer when administered to the basolateral side of differentiated Caco2 cells and the effects can be partially inhibited by monoclonal antibodies against IL1, IL6 and IL8. At nanomolar concentrations WGA stimulates the synthesis of pro-inflammatory cytokines and thus the biological activity of WGA should be reconsidered by taking into account the effects of WGA on the immune system at the gastrointestinal interface. These results shed new light onto the molecular mechanisms underlying the onset of gastrointestinal disorders observed in vivo upon dietary intake of wheat-based foods."

    Effects of wheat germ agglutinin on human gastrointestinal epithelium: Insights from an experimental model of immune/epithelial cell interaction. Toxicology and Applied Pharmacology. June 1, 2009 v237 i2 p146(8). Authors: Chiara Dalla Pellegrina (a), Omar Perbellini (b), Maria Teresa Scupoli (b)(c), Carlo Tomelleri (b), Chiara Zanetti (a), Gianni Zoccatelli (a), Marina Fusi (a), Angelo Peruffo (a), Corrado Rizzi (a), Roberto Chignola (a)
    Yes, that sounds like what it is saying to me.

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    jqbancroft's Avatar
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    Thanks Katt! I thought that's what they were trying to say, but I needed more structural hints like "while others have said ____, our research indicates that______"

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